|
E. coli
|
| EC100λpir |
supE44 ΔlacU169 (ϕ80 lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 (λpirR6K) |
Epicenter |
| SM10λpir |
thi-1 thr leu tonA lacY supE recA: :RP4-2-Tc: :Mu kmR (λpirR6K)
|
Lab collection |
| BW25113 |
F
− Δ(araD-araB)567 lacZ4787Δ: :rrnB-3 LAM
−
rph-1 Δ(rhaD-rhaB)568 hsdR514
|
Baba et al. (2006)
|
| ER2566 |
fhuA2 lacZ: :T7 gene1 [lon] ompT gal sulA11 R(mcr-73: :miniTn10–TetS)2 [dcm] R(zgb-210: :Tn10–TetS) endA1 Δ(mcrCmrr)114: :IS10 |
New England BioLabs |
|
|
|
V. cholerae
|
| JB58 |
V. cholerae O1 El Tor strain N16961 ΔlacZ, SmR
|
Lab collection |
| XBV222 |
JB58ΔleuO
|
Bina et al. (2013)
|
| XBV148 |
JB58ΔaphB
|
This study |
| JB804 |
V. cholerae O1 El Tor strain C6706, SmR
|
Thelin & Taylor (1996)
|
| XBV144 |
JB804 ΔlacZ
|
This study |
| VA412 |
XBV144 ΔleuO
|
This study |
| EC20568 |
C6706 Tn: :VC2485 (leuO) |
Cameron et al. (2008)
|
| EC17926 |
C6706 Tn: :VC0278 (cadA) |
Cameron et al. (2008)
|
|
|
|
Plasmid
|
| pTL61T |
lacZ transcriptional reporter plasmid, CbR
|
Linn & St Pierre (1990)
|
| pXB239 |
pTL61T containing the cadC promoter region |
This study |
| pXB203 |
pTL61T containing the aphB promoter region |
Bina & Bina (2010)
|
| pBAD18 |
Arabinose-regulated expression plasmid, CbR
|
Guzman et al. (1995)
|
| pVA94 |
pBAD18 expressing leuO
|
Ante et al. (2015)
|
| pBAD18Km |
Arabinose-regulated expression plasmid, KmR
|
Guzman et al. (1995)
|
| pXB298 |
pBAD18Km expressing leuO
|
Bina et al. (2013)
|
| pBAD33 |
Arabinose-regulated expression plasmid, CmlR
|
Guzman et al. (1995)
|
| pVA126 |
pBAD33 expressing leuO
|
This study |
| pWM91 |
Suicide plasmid vector used for allelic exchange |
Metcalf et al. (1996)
|
| pDLT |
pWM91 containing a fragment of lacZ harbouring an internal deletion |
Fullner & Mekalanos (1999)
|
| pWM91ΔleuO
|
pWM91 containing a fragment of leuO harbouring an internal deletion |
Moorthy & Watnick (2005)
|
| pWM91ΔaphB
|
pWM91 containing a fragment of aphB harbouring an internal deletion |
This study |
| pMAL-c2 |
IPTG-inducible expression vector for fusion of proteins to MBP and cytoplasmic expression, CbR
|
New England BioLabs |
| pVA175 |
pMAL-c2 expressing leuO
|
This study |
|
|
|
Oligonucleotide
|
|
|
| PcadC-F |
TTCTCGAGTCGGGCTATCGACTGTACGATG |
|
| PcadC-R |
GTTCTAGACACCACACACCGATGAAGAGCGAAATTATAA |
|
| aphB-F1 |
TTGGATCCGCCCCACGATGGCTCGCG |
|
| aphB-F2 |
CGACTGGTTGTCACAAAGATCACCAGCCGGAAAAAGTGCGCCTG |
|
| aphB-R1 |
GCGAGCTCCAGTGGGCGATATGGGCG |
|
| aphB-R2 |
GGTGATCTTTGTGACAACCAGTCGAAAGAGGTTTAGGTCATCTAG |
|
| LeuO-F |
CCCCCGGGTTAGATAAAAAAGACGCAATGAGTGCC |
|
| LeuO-R |
CCTCTAGATAGAAACGTAGAATGAACAAAGGATC |
|
| cadC-EMSA-F1 |
GCGGGAGTCGGCAGCGGATGGTTAAACAACCTAAGTT |
|
| cadC-EMSA-R1 |
GCGGGAGTCGGCAGCGGAGCGAAATTATAAGTGCAC |
|
| cadC-EMSA-F2 |
GCGGGAGTCGGCAGCGAATTTCGCTCTTCATCGGTG |
|
| cadC-EMSA-R2 |
GCGGGAGTCGGCAGCGCATAGAATAGCTCTTTGTATC |
|
| 5′BIO |
GCGGGAGTCGGCAGCG |
|
