Table 1. Primers used in this study.
Gene | Primer sequence used for cloning (5′–3′)* | Primer sequence used for qPCR (5′–3′) |
GTPCH-1 | CGGAATTCATGGCAAGCAGCATCGAGAAC | GACGGCCTTAGCTTCCCAAG |
GTPCH-1 | CCGCTCGAGCTAAATCCCACTGCGTCTGATAAG | CGAACTGCCTCTGCAATCCT |
GTPCH-2 | CGGAATTCATGTCCCACACTCCCACC | TCACGAAGCAAGTCGCAATG |
GTPCH-2 | CCGCTCGAGCTACACGCCACGTCGTCTG | TGATCAAGGACAAGAACTCCTCC |
PTPS | CGGAATTCATGACATCCTCAACACCCGTC | TGACATCCTCAACACCCGTCAG |
PTPS | CCGCTCGAGTTATTCTCCTCGGTAAACAACG | GCATTTGCCAAAGAGCTTCAC |
SR | CTAGCTAGCATGTCGTCCAAGGAGCATC | CTTGGCAGGAGGCTAGAAGTTAC |
SR | CCCAAGCTTCTACTCATCATAAAAGTCGATGTGT | ACGGCGAGCAGTGAGGATAT |
18S rDNA | − | CTATTGGCGGAGGTCTATTCGT |
18S rDNA | − | GCACGCATTCGGATAATTGGT |
Restriction sites are underlined.