Skip to main content
. 2011 Nov;157(Pt 11):3059–3070. doi: 10.1099/mic.0.051847-0

Table 1. Primers used in this study.

Gene Primer sequence used for cloning (5′–3′)* Primer sequence used for qPCR (5′–3′)
GTPCH-1 CGGAATTCATGGCAAGCAGCATCGAGAAC GACGGCCTTAGCTTCCCAAG
GTPCH-1 CCGCTCGAGCTAAATCCCACTGCGTCTGATAAG CGAACTGCCTCTGCAATCCT
GTPCH-2 CGGAATTCATGTCCCACACTCCCACC TCACGAAGCAAGTCGCAATG
GTPCH-2 CCGCTCGAGCTACACGCCACGTCGTCTG TGATCAAGGACAAGAACTCCTCC
PTPS CGGAATTCATGACATCCTCAACACCCGTC TGACATCCTCAACACCCGTCAG
PTPS CCGCTCGAGTTATTCTCCTCGGTAAACAACG GCATTTGCCAAAGAGCTTCAC
SR CTAGCTAGCATGTCGTCCAAGGAGCATC CTTGGCAGGAGGCTAGAAGTTAC
SR CCCAAGCTTCTACTCATCATAAAAGTCGATGTGT ACGGCGAGCAGTGAGGATAT
18S rDNA CTATTGGCGGAGGTCTATTCGT
18S rDNA GCACGCATTCGGATAATTGGT
*

Restriction sites are underlined.