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The data shown are the mean of three independent experiments.
| Enzyme | Substrate | Km (mM) | Vmax (µM min−1) |
| GTPCH-1 | GTP | 0.88±0.04 | 0.145±0.006 |
| GTPCH-2 | GTP | 0.37±0.02 | 0.408±0.004 |
| PTPS | H2-NTP | 0.164±0.005 | 4.61±0.21 |
| SR | Sepiapterin | 2.14±0.12 | 15.93±0.75 |
| SR | NADPH | 0.303±0.004 | 13.05±0.05 |
