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. Author manuscript; available in PMC: 2017 Apr 15.
Published in final edited form as: Mol Cell Endocrinol. 2015 Dec 28;425:48–60. doi: 10.1016/j.mce.2015.12.006

Figure 4. The effect of siRNA silencing targeting CK2 holoenzyme (CK2α+α’+β) on leucine deprivation-induced IGFBP-1 phosphorylation.

Figure 4

HepG2 cells were treated with scrambled or CK2α+α’+β siRNA for 24 hours and incubated in leucine plus (450 μM) or leucine deprived (0 μM) media for 72 hours (n=3 each). Representative immunoblots of conditioned HepG2 cell media (50 μL per well) treated with scrambled, or CK2α+α’+β siRNA in media that was leucine plus or leucine deprived assessed for A. total IGFBP-1 and B. IGFBP-1 phosphorylation at Ser101, Ser119 and Ser169. Knockdown of the CK2 holoenzyme prevents IGFBP-1 hyperphosphorylation in leucine deprivation. Values are displayed as mean + SEM. *p< 0.05, **p= 0.001-0.05, ***p < 0.0001 versus control; One-way analysis of variance; Dunnet’s Multiple Comparison Test; n=3. Sc: Scrambled, 450 μM leucine. Sc:0: Scrambled, 0 μM leucine. CK2:450: CK2α+α’+β siRNA, 450 μM leucine. CK2:0: CK2α+α’+β siRNA, 0 μM leucine.