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. Author manuscript; available in PMC: 2017 Apr 15.
Published in final edited form as: Mol Cell Endocrinol. 2015 Dec 28;425:48–60. doi: 10.1016/j.mce.2015.12.006

Figure 6. MRM/MS analysis for assessment of IGFBP-1 phosphorylation.

Figure 6

Mass spectrometry analysis of the relative IGFBP-1 phosphorylation induced by leucine deprivation in the presence or absence of TBB or BIS. TBB or BIS attenuate IGFBP-1 phosphorylation at A. Ser101, B. Ser119 and C. Ser169 in the presence of either inhibitor (TBB samples (set to a value of 1) and normalized to a non-phosphorylated peptide within the IGFBP1 protein. C:450 Control, 450 μM leucine. C:0: Leucine deprivation, 0 μM leucine. BIS:0: Bisindolylmaleimide (7.5 μM), 0 μM leucine. TBB:0: TBB (1 μM), 0 μM leucine.