Figure 4.

Time course of mitochondrial function in the mouse heart after PO. In A–E, the values from sham-operated mouse hearts at each time point were averaged and expressed as 1. * p<0.05 vs. sham at each time point. In A–B, C57BL/6J mice were subjected to either sham operation (n=34) or TAC for 6 or 24 hours, or 2, 3, 5, 7, 14 or 30 days (n=4, 4, 4, 4, 4, 6, 4, and 4, respectively). A. Relative mitochondrial ATP production in the mouse heart. *¹ p=0.021, *² p=0.027, *³ p=0.009, *⁴ p=0.025. B. Relative mitochondrial ATP content in the mouse heart. *¹ p=0.036, *² p=0.029, *³ p=0.001, *⁴ p=0.049, *⁵ p=0.043. In C–E, C57BL/6J mice were subjected to either sham operation (n=29) or TAC for 6 or 24 hours, or 2, 3, 5, 7, 14 or 30 days (n=4, 4, 3, 4, 3, 3, 4, and 4, respectively). C. Relative respiratory chain complex I activity in mitochondria. *¹ p=0.043, *² p=0.032, *³ p=0.007, *⁴ p=0.022. D. Relative respiratory chain complex II+III activity in mitochondria. *¹ p=0.022, *² p=0.003, *³ p=0.004. E. Relative respiratory chain complex IV activity in mitochondria. *¹ p=0.014, *² p=0.012, *³ p=0.014, *⁴ p=0.002. In F–H, mice were subjected to either sham operation (n=5) or 14 days of TAC (n=4). The mitochondrial fraction prepared from heart homogenates was subjected to Seahorse analyses. * p<0.05 vs. sham at 14 days. F. Basal respiration.* p=0.013 vs. sham at 14 days. G. Maximal respiration.* p=0.006 vs. sham at 14 days. H. Proton leak.* p=0.004 vs. sham at 14 days.