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. Author manuscript; available in PMC: 2017 Apr 1.
Published in final edited form as: Acta Biomater. 2015 Oct 20;34:60–72. doi: 10.1016/j.actbio.2015.10.030

Fig. 7.

Fig. 7

3D combinatorial screening from a modular materials library. (a) Enzymatically mediated cross-linking scheme, where xxxx xxxx represents specific peptide sequence. (b) Components of the combinatorial toolbox are assembled from biologically relevant factors in categorized form. Stiffness and MMP sensitivity of the matrix are set within the experimentally measured ranges shown (n = 3 replicates). (c) Experimental process consists of combining the components library with reporter cells using robotic mixing and dispensing technology into 1536-well plates (n = 3 replicates). (d–f) Automated microscopy and image processing to determine colony size and GFP intensity. Average cell density per well is set by the initial cell concentration used in the experiment, and exact initial cell density for each well is determined retrospectively by imaging. Examples of a set of images tracking colony growth in a single well over the course of a 5-day experiment (d) 3D confocal reconstruction (e) and image segmentation are shown (f). Scale bar, 200 mm. (Figure from Ref. [97] with permission).