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. Author manuscript; available in PMC: 2017 Apr 1.
Published in final edited form as: Clin Lymphoma Myeloma Leuk. 2015 Dec 23;16(4):203–212. doi: 10.1016/j.clml.2015.12.005

Figure 4. MYXV initiates apoptosis through activation of Casp-8.

Figure 4

(A) Human U266 MM cells were either mock-treated or infected with MYXV at an MOI=10. At the indicated times post-infection, cells were lysed and the activity of both Casp-8 and Casp-9 were measured using Caspase-Glo™ analysis. Data is presented as the ratio of signal from mock-treated and infected cells. (B) Human U266 MM cells were pretreated with the indicated caspase inhibitors for 30 minutes and then either mock-treated or infected with MYXV at an MOI=10. Six hours post-infection, cells were harvested and samples were separated on a SDS-PAGE gel. The presence and cleavage status of both Casp-8 and Casp-9 was then determined using immunoblot.