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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: J Endocrinol. 2015 Aug 18;227(1):13–24. doi: 10.1530/JOE-15-0098

Figure 1. Effect of STAT4 deficiency on lymphocyte numbers and relative composition in visceral and peri-aortic adipose tissue.

Figure 1

(A): CD45+ cells in peri-aortic and visceral adipose tissues were determined by flow cytometry and normalized to tissue weight; * = significant compared to visceral adipose tissue for the same mice group. Also shown is a representative gating strategy for the forward scattered and CD45+ gates in Apoe-/- mice. (B): Immunohistochemistry for CD45 in the peri-aortic and visceral fat of Stat4-/-Apoe-/- and Apoe-/- mice. Relative percentages of CD3+, CD3+CD4+, CD3+CD8+ and NK40.6+ were determined in the CD45+ gate in peri-aortic (C) and visceral (D) adipose tissues of mice on western diet. Representative FACS plots in visceral fat are also shown. Data is from n=5-7 mice/group.