Protocol 1 Western Blot Antibody | ||||
|---|---|---|---|---|
| POI | Loading Control | |||
| Independent Gels | Description | Working Conc. | Description | Working Conc. |
| 1 | Mouse anti-pERK1/2 (T202/Y204) (42, 44kDa) | 1:1000 | Rabbit anti-MEK1/2 (45 kDa) | 1:1000 |
| 2 | Rabbit anti-pMEK1/2 (S217/221) (45 kDa) | 1:1000 | Mouse anti-ERK1/2 (42, 44 kDa) | 1:1000 |
| 3 | Rabbit anti-MAP3K8 (52, 58 kDa) | 1:500 | Mouse anti-ß-Actin (43 kDa) | 1:100 – 1:1000 |
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