| Reagent | Type | Manufacturer | Catalog # | Comments |
|---|---|---|---|---|
| RPMI medium with L-glutamine | Cell culture | Sigma | R8758 | Replaces Corning cat no. 10-040-CV. |
| MEM with L-glutamine | Cell culture | Sigma | M4655-500ML | Replaces Corning cat. no. 10-010-CV. |
| FBS | Cell culture | Life Technologies | 12483-020 | Replaces Corning brand. |
| Pen/strep/glutamine | Cell culture | Abm | G255 | Replaces Corning brand. |
| A375 cells | Cell line | ATCC | CRL-1619 | Original brand not specified. |
| RPMI-7951 cells | Cell line | ATCC | HTB-66 | |
| OUMS-23 cells | Cell line | JCRB | JCRB1022 | Original brand not specified. |
| PBS | Buffer | Sigma | D8537-500ML | Original brand not specified. |
| Trypsin | Cell culture | Sigma | T4049 | Original brand not specified. |
| 10 cm plates | Labware | CellStar | 664 160 | Original brand not specified. |
| 96 well clear plates | Labware | Sarstedt | 83.3924 | Original brand not specified. |
| WST1 viability assay | Reporter assay | Roche | 11644807001 | Original catalog # not specified. |
| Microplate reader (420–480 nm) | Instrument | Molecular Devices | abm | Original brand not specified. |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.