Reagent	Type	Manufacturer	Catalog #	Comments
RPMI medium with L-glutamine	Cell culture	Sigma	R8758	Replaces Corning cat no. 10-040-CV.
FBS	Cell culture	Life Technologies	12483-020	Replaces Corning brand.
Pen/strep/glutamine	Cell culture	Abm	G255	Replaces Corning brand.
A375 cells	Cell line	ATCC	CRL-1619	Original brand not specified.
PBS	Buffer	Sigma	D8537-500ML	Original brand not specified.
Trypsin	Cell culture	Sigma	T4049	Original brand not specified.
10 cm plates	Labware	CellStar	664 160	Original brand not specified.
6 well plates	Labware	Greiner bio-one	657 160	Original brand not specified.
Polybrene	Cell culture	Sigma	H9268	Original brand not specified.
Blasticidin	Cell culture	Invivogen	ant-bl-1	Original brand not specified.
PLX4720	Inhibitor	Selleck Chemicals	S1152	Replaces Symansis brand.
AZD6244	Inhibitor	Selleck Chemicals	S1008	Original catalog # not specified.
CI-1040	Inhibitor	Selleck Chemicals	S1020	Replaces Shanghai Lechen International Trading Co. brand.
DMSO	Chemical	Sigma	D4540	Original brand not specified.
NP-40 buffer	Buffer	Life technologies	FNN0021	Original brand not specified.
Protease inhibitors	Inhibitor	Roche	04693116001	Original catalog # not specified.
Phosphatase inhibitor cocktail I	Inhibitor	Sigma	P2850	Replaces CalBioChem brand.
Phosphatase inhibitor cocktail II	Inhibitor	Sigma	P5726	Replaces CalBioChem brand.
Cell scraper	Labware	Sasrstedt	83.1830	Original brand not specified.
BCA kit	Reporter assay	Pierce	23227	Original catalog # not specified. Communicated by authors.
DTT	Chemical	Biobasic	DB0058	Original brand not specified.
Sample buffer	Buffer	abm	G031	Replaces Invitrogen brand.
Protein molecular weight ladder	Western materials	abm	G252, G494	Original brand not specified.
10% Tris/Glycine gel; 10 well, 1.0 mm thick	Western materials	abm	internal	Replaces Invitrogen brand.
Running buffer	Buffer	abm	internal	Original brand not specified.
Immobilon P	Western materials	Thermofisher	IPVH00010	Original brand not specified.
Transfer buffer	Buffer	abm	internal	Original brand not specified.
Mouse anti-pERK1/2 (T202/Y204) (clone E10) antibody	Antibodies	Cell Signaling	9106	Use at 1:1000 dilution. Original catalog # not specified.
Mouse anti-p44/42 MAPK (ERK1/2) (clone L34F12) antibody	Antibodies	Cell Signaling	4696	Use at 1:11000 dilution. Replaces catalog # 4695. Communicated by authors.
Mouse anti-V5-HRP conjugated antibody	Antibodies	Invitrogen	R961-25	Use at 1:5000 dilution. Original catalog # not specified.
Rabbit anti-Vinculin antibody	Antibodies	Sigma	V4139	Use at 1:20,000 dilution. Original catalog # not specified.
Rabbit anti-MEK1/2 (clone D1A5) antibody	Antibodies	Cell Signaling	8727	Use at 1:1000 dilution. Original catalog # not specified.
Rabbit anti-MAP3K8 (clone M-20) antibody	Antibodies	Santa Cruz	sc-720	Use at 1:500 dilution. Communicated by authors.
Anti-rabbit IgG – HRP conjugated antibody	Antibodies	Cell Signaling	7074	Use at 1:1000 dilution. Original catalog # not specified.
Anti-mouse IgG – HRP conjugated antibody	Antibodies	Cell Signaling	7076	Use at 1:1000 dilution. Original catalog # not specified.
Chemiluminescent reagent	Western materials	Life Technologies	WP20005	Replaces Pierce brand.