Figure 7. MGL1 targeting with OVA-LeX shows sustained antigen presentation in MHC-I.
WT BM-DCs were pulsed for 4 h with titrated amounts of OVA-LeX and washed with culture medium. DCs were then chased for 48 h in antigen-free medium. (A) BM-DCs pulsed for 4h with OVA-LeX induced MHC-I antigen presentation as measured by CFSE-labeled OVA-specific OT-I cells (upper panel). Sustained presentation is shown after 48 h (lower panel). (B) MHC-II antigen presentation 4h and 48h after pulse-loading with OVA-LeX, analyzed by OT-II proliferation. Data are presented as percentage of proliferated T cells and representative of three independent experiments.