(
A) Log-log MSD plot of the
FBA1 mRNP after treatment as described in
Figure 2C. (
B) Log-log MSD plot of the
URA3 locus after treatment as described in
Figure 2C. (
C) Log-log MSD plot of the pLacO plasmid after treatment as described in
Figure 2C. (
D) Graphic depicting the quantification of intranuclear displacements between the
PES4 locus (green) and the
POA1 locus (red). (
E) Log-log interchromosomal MSDs quantifying the displacement between
POA1 and
PES4 during logarithmic growth, (+) glucose, and after acute starvation, (–) glucose. Images were acquired every 1.5 s for a total of 150 s, and the intranuclear displacements between the
POA1 locus and the
PES4 locus were tracked over time by determining the absolute distance between the two loci. (
F) Log-log interchromosomal MSDs quantifying the displacement between
POA1 and
PES4 after inhibition of the cytoskeleton (nocodazole + LatA) and in a DMSO-treated control. Cells were treated as described in
Figure 2C and imaged as in (
E). Dashed gray lines represent a slope of one to guide the eye.