Figure 2. NIFK promotes the migration of lung cancer cells in vitro.

(A) The endogenous expression levels of NIFK in lung adenocarcinoma (Left), squamous and large cell lung cancer cell lines (Right). The relative expression levels were normalized to those of normal Beas2B lung cells, and the average expression levels are presented. (B) The relative NIFK levels in A549 and PC13 cells after overexpression of NIFK via lentiviral infection. (C) The migratory capacity of NIFK-overexpressing A549 and PC13 cells was assessed using a wound-healing assay. The exposed area was measured after the indicated incubation period and was normalized to that of the 0-h control. (D) The NIFK knockdown efficiencies in the lentivirus-based shRNA clones sh5 and sh6, corresponding to H661 and H1299 cells, respectively. NS, non-silenced control. (E) H661 and H1299 cell migration after NIFK knockdown was evaluated at the indicated time points.

DOI: http://dx.doi.org/10.7554/eLife.11288.005

Figure 2—figure supplement 1. The overexpression of NIFK promotes cell migration, invasion in vitro and metastasis in vivo.

(A-B) Cell migration and invasion were evaluated via the transwell assay. A549 cells and PC13 cells were incubated for 4.5 hr and 24 hr, respectively, to evaluate cell migration. For the invasion assay, both A549 and PC13 cells were incubated for 42 hr.

DOI: http://dx.doi.org/10.7554/eLife.11288.006