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. 2016 Mar 30;7:367. doi: 10.3389/fpls.2016.00367

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Copyright © 2016 Sun, Li, Xiong, Zhao and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Non-saturating, saturating and “release-on-demand” dsDNA intercalating dyes. (A) At non-saturating concentrations the dye quickly rebinds to regions that remain double stranded, consequently there is no drop in fluorescence. (B,C) Saturating and “release-on-demand” dyes do not redistribute from the melted regions of single-stranded DNA back to dsDNA, resulting in a reduction of fluorescence. This difference gives dyes such as SYT09 or EvaGreen the high sensitivity required for HRM analysis.