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E11.5 first branchial arches cultured with MetBIO (control) or BIO (40 μM) for 24 h. In situ hybridization for Lef1 (A,B), Msx1 (C,D), Fgf8 (E,F), Epfn (G,H), and Shh (I,J) mRNA. The expression of these genes increased and extended to the distal region of branchial arches in BIO treatment samples, when compared to controls. Arrows and arrowheads indicate the presumptive molar and incisor region, respectively. Scale bar: 200 μm.
