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. 2016 Mar 30;4:25. doi: 10.3389/fcell.2016.00025

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Copyright © 2016 Aurrekoetxea, Irastorza, García-Gallastegui, Jiménez-Rojo, Nakamura, Yamada, Ibarretxe and Unda.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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E14.5 first molars cultured with MetBIO (control) or BIO (20 μM) for 6 days. Control molar sections stained with hematoxylin-eosin (A) had normal development of dental cusps, clear polarization of the inner dental epithelia cells (ide), odontoblast differentiation (od), and a thin layer of predentin (pd). In contrast, molars cultured with BIO treatment (B) showed an irregular and disorganized dental cusp pattern, non-polarized cells in the inner dental epithelium and an undifferentiated odontoblastic layer. Alkaline phosphatase activity spread all over the dental mesenchyme in BIO-treated molars (D), but in control samples, enzymatic activity was detected in only in the odontoblastic and subodontoblastic layers (C). The dotted region corresponds to dental epithelium. Od, odontoblasts; ide, inner dental epithelium; pd, predentin. Scale bar: 200 μm.