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. 2016 Mar 30;4:25. doi: 10.3389/fcell.2016.00025

Figure 7.

Figure 7

MetBIO (control) and BIO (20 μM) treatment of E17.5 and E19.5 first molars for 6 or 4 days. Whole molar cultures analyzed by stereoscopic microscopy (A–D). Frontal sections of molars stained with hematoxylin-eosin (E–H′). These pictures show striking differences in the growth of the inner dental epithelium and in odontoblast differentiation of control (E,E′, G,G′) and BIO-treated (F,F′, H,H′) samples. Immunofluorescence for Nestin (I–L′) and Amelogenin (M–P′) in control and BIO-treated molars. Nestin labeled odontoblasts and spread to the subodontoblastic area (arrow, J,J′) in E17.5 BIO-cultures, whereas in the controls (I,I′), NNestin was restricted to the odontoblastic layer. In E19.5 cultures, both controls and treatments showed Nestin in odontoblasts (K,L′). The presence of amelogenin significantly decreased (N,N′) in E17.5 BIO-treated inner dental epithelium (N′, arrow) compared to controls (M,M′, arrow). E19.5 cultures treated with BIO (P,P′) exhibited slightly reduced amelogenin expression compared to the controls (O,O′). am, ameloblasts; od, odontoblasts; pd/d, predentin/dentin. Scale bar: 200 μm.