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. 2016 Mar 30;7:382. doi: 10.3389/fmicb.2016.00382

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Copyright © 2016 Ma, Geudens, Kieu, Sinnaeve, Ongena, Martins and Höfte.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Swarming motility of wild type strain Pseudomonas sp. CMR5c and deletion mutants in orfamide biosynthesis (CMR5cΔofa) or in orfamide regulatory genes (CMR5cΔluxRup and CMR5cΔLuxRdown). In all mutants swarming was restored by chemical complementation with orfamide B. Representative plates are shown and the diameter of soft agar plates used in this assay is 9 cm. The insert shows the UPLC-MS analysis of wild type and mutant strains. (B) Swarming motility of P. protegens CHA0, P. protegens Pf-5, Pseudomonas sp. CMR5c, Pseudomonas sp. CMR12a, and its sessilin mutant CMR12a-Clp1.