Table 2.
Putative orfamide-synthases and flanking region identified from P. protegens and related strains in this study.
| Orfamide-synthases and flanking area | Identity at amino acid level | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| P. protegens | Pseudomonas sp. | ||||||||
| CHA0 | Cab57 | Pf-5 | Wayne1R | CMR5c | CMR12a | CMAA1215 | PH1b | ||
| 1a | GCN5-related N-acetyltransferase | [100%] | [75%] | [100%] | |||||
| ALG76232.1 | AFH75326.1 | ERO60965.1 | |||||||
| 1b | RND efflux system, outer membrane lipoprotein | 100% | 99% | 99% | 99% | 87% | |||
| AGL83955.1 | BAO61507.1 | AAY91416.1 | WP_019094693.1 | WP_025130134.1 | |||||
| 2 | LuxR family transcriptional regulator | 100% | 99% | 100% | 99% | 80% [100%] | 78% [82%] | 80% [100%] | 88% [80%] |
| AGL83956.1 | BAO61508.1 | AAY91417.1 | WP_011060444.1 | ALG76233.1 | AFH75327.1 | ERO60966.1 | WP_025130133.1 | ||
| ofaA | Nonribosomal peptide synthase | 100% | 99% | 99% | 99% | 80% [100%] | 79% [84%] | 80% ERO60967.1 | 83% [80%] |
| AGL83957.1 | BAO61509.1 | AAY91419.3 | WP_019094694.1 | ALG76234.1 | AFH75328.1 | +78% ERO60968.1 | WP_025130132.1 | ||
| +87% ERO60969.1 | |||||||||
| ofaB | Nonribosomal peptide synthase | 100% | 99% | 99% | 99% | 81% [100%] | 82% [89%] | 82% [99%] | 85% [80%] |
| AGL83958.1 | BAO61510.1 | AAY91420.2 | WP_037007783.1 | ALG76235.1 | AFH75329.1 | ERO60970.1* | WP_025130131.1 | ||
| ofaC | Nonribosomal peptide synthase | 100% | 100% | 99% | 98% | 82% [100%] | 82% [88%] | 80% ERO60971.1 | 86% [82%] |
| AGL83959.1 | BAO61511.1 | AAY91421.3 | WP_019095728.1 | ALG76236.1 | AFH75330.1 | +ERO60972.1* | WP_025130130.1 | ||
| 3 | Macrolide efflux protein MacA | 100% | 99% | 99% | 99% | 93% [100%] | 92% [97%] | 93% [100%] | 98% [93%] |
| AGL83960.1 | BAO61512.1 | AAY91422.1 | WP_011060449.1 | ALG76237.1 | AFH75331.1 | ERO60973.1 | WP_025130129.1 | ||
| 4 | Macrolide efflux protein MacB | 100% | 99% | 99% | 99% | 93% [100%] | 91% [97%] | 96% [95%] | 97% [93%] |
| AGL83961.1 | BAO61513.1 | AAY91423.1 | WP_019095727.1 | ALG76238.1 | AFH75332.1 | ERO60957.1* | WP_025130128.1 | ||
| 5 | LuxR family transcriptional regulator | 100% | 99% | 99% | 99% | 78% [100%] | 74% [81%] | 78% [100%] | 78% [77%] |
| AGL83962.1 | BAO61514.1 | AAY91424.1 | WP_019095116.1 | ALG76239.1 | AFH75333.1 | ERO60958.1 | WP_025130127.1 | ||
| 6 | Flagellar basal body rod modification protein FlgD | [100%] | 99% | ||||||
| ALG76240.1 | ERO60959.1 | ||||||||
| 7 | Flagellar hook protein FlgE | [100%] | 99% | ||||||
| ALG76241.1 | ERO60960.1 | ||||||||
| 8 | Putative gnat family acetyltransferase | 100% | 100% | ||||||
| AGL83963.1 | BAO61515.1 | ||||||||
| 9 | Family 2 glycosyl transferase | 100% | 100% | ||||||
| AGL83964.1 | BAO61516.1 | ||||||||
| 10 | Hypothetical protein | AGL83965.1 | |||||||
| 11 | Hypothetical protein | WP_029978544.1 | |||||||
| 12 | Glyoxalase family protein | 100% | 100% | 100% | 99% | 82% [100%] | 79% [90%] | 82% [100%] | 91% [80%] |
| AGL83966.1 | BAO61517.1 | AAY91425.1 | WP_019095115.1 | ALG76242.1 | AFH75334.1 | ERO60961.1 | WP_025130125.1 | ||
| 13 | Heme transporter CcmD, radical SAM domain-containing protein | 100% | 99% | 99% | 99% | 93% [100%] | 92% [95%] | 93% [99%] | 97% [93%] |
| AGL83967.1 | BAO61518.1 | AAY91426.1 | WP_026020220.1 | ALG76243.1 | AFH75335.1 | ERO60962.1 | WP_025130124.1 | ||
The identity level of amino acids was compared by BLASTp search. The first BLASTp similarity comparison is based on protein sequence of P. protegens CHA0. The second BLASTp similarity comparison is based on Pseudomonas sp. CMR5c and the level of similarity is shown in brackets.
*
Partial NRPS sequences were retrieved from Pseudomonas sp. CMAA1215 in GenBank database.