Table 3.
Percent mortality of brine shrimps (Artemia salina) larvae at 26°C after treatment with toxin (AFB1 and AFB2) detoxified with T. ammi seeds extract at various incubation periods.
| Treatments | Incubation period (h) | No. of living shrimps | No. of dead shrimps | % Mortality |
|---|---|---|---|---|
| CONTROL | ||||
| Sea water + shrimps | 24 48 72 96 |
40 40 40 39 |
0 0 0 1 |
0 0 0 2.5 |
| Methanol + shrimps | 24 48 72 96 |
38 38 37 36 |
1 2 3 3 |
2.5 5 7.5 7.5 |
| Untreated toxins + shrimps | 24 48 72 96 |
7 5 4 3 |
33 35 36 37 |
83.0 86.7 89.2 91.7 |
| TREATMENT | ||||
| Treated toxin with T. ammi seeds extract + shrimps | 24 48 72 96 |
35 34 32 31 |
5 6 8 9 |
11.7 15.0 20.0 23.3 |
Sea water was taken as a control. Other Controls consist of Methanol and untreated toxin AFB1 (100 ng/ml) and AFB2 (50 ng/ml) dried and redissolved in sea water.
Values are mean of three replicates.
Data were analyzed by analysis of Variance (ANOVA).