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. 2016 Feb 13;5(4):305–316. doi: 10.1016/j.molmet.2016.02.001

Figure 3.

Figure 3

Role of NFκB in palmitate-induced CSF3 expression. (A) The mRNA expression of the indicated genes was measured in human myotubes treated for 24 h with palmitate (0.5 mmol/L) or stearate (0.5 mmol/L), respectively. (B–F) Human myotubes were pretreated for 24 h with the indicated siRNA prior to 24-hour treatment with palmitate (0.5 mmol/L), and knockdown of the target gene and its influence on CSF3 mRNA expression was assessed by RT-PCR. Data are given as means ± SEM. Two-group comparisons were performed using matched-pairs Student's t-test (N ≥ 3; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).