Table 1.

LC gradient conditions.

#	Time [min]	H2O [%]	ACN [%]	Buffer [%]	Flow rate [μL/min]
0	0	0	95	5	300
1	0.5	0	95	5	300
2	20	25	70	5	300
3	24	45	50	5	300
4	26	75	20	5	300
5	29	75	20	5	300
6	29.1	0	95	5	400
7	33.9	0	95	5	400
8	34	0	95	5	50
9	—	100	0	0	50