Table 6.
Expression difference of genes putatively involved in glucosylation and acylation of flavonoids in Brassica oleracea.
| Enzyme step | Brassica oleracea predicted genes | Gene abbreviation | Microarray element | Light Log2-difference LL-HL | Temperature Log2-difference LT-HT |
|---|---|---|---|---|---|
| Flavonol 3-O-GT Flavonol 7-O-GT UGT73B | Bo1g007620 | UGT73B2/3 | JCVI_35049 | −2.33 | −0.49 |
| Bo1g007630 | UGT73B2/3 | n | |||
| Bo3g169080 | UGT73B2/3 | JCVI_28429 | −2.53 | 0.59 | |
| Bo3g169090 | UGT73B2/3 | JCVI_3139 | −2.00 | −1.77 | |
| Quercetin 3-O-GT Flavonol 7-O-GT UGT76E | Bo2g026640 | UGT76E1 | ES942112 | 0.49 | 0.4346 |
| Bo9g139510 | UGT76E2 | JCVI_42206 | −0.38 | 0.4533 | |
| Bo3g132780 | UGT76E11/12 | JCVI_33139 | 1.01 | 0.6658 | |
| Bo1g070870 | UGT76E11/12 | CA992143 | 0.28 | 0.9358 | |
| Flavonol 3-O-GT UGT78D | no gene present | UGT78D2 | n | ||
| Bo3g003590 | UGT78Drelated | JCVI_6551 | 0.40 | −1.45 | |
| Flavonol 7-O-GT UGT73C | Bo3g030310 | UGT73C5/6 | EV108354 | −3.16 | 2.93 |
| Bo4g031140 | UGT73C5/6 | EV108083 | −2.03 | 4.31 | |
| Bo4g031150 | UGT73C5/6 | JCVI_27911 | −2.67 | 4.58 | |
| Sinapate 1-GT | Bo1g105850 | UGT84A2 | JCVI_9170 | −2.38 | 1.43 |
| Bo5g100950 | UGT84A2 | n | |||
| Bo1g056800 | UGT84A1 | n | |||
| Bo7g103790 | UGT84A1 | JCVI_13790 | 1.26 | 0.23 | |
| Bo1g056770 | UGT84A3 | JCVI_15448 | −2.76 | 0.32 | |
| Bo1g056740/50/60 | UGT84A4 | n/n/n | |||
| Acyl-glucose dependent glucosyltransferase (BGLU) | Bo3g018610 | BGLU1-6 | EV102326 | −1.21 | 1.53 |
| Bo3g018620 | BGLU1-6 | JCVI_28076 | −2.27 | 1.66 | |
| Bo3g039750 | BGLU1-6 | JCVI_25479 | −1.07 | 2.28 | |
| Bo4g199780 | BGLU1-6 | JCVI_28076 | −2.27 | 1.66 | |
| Bo6g089150 | BGLU1-6 | JCVI_34674 | −2.29 | −0.0696 | |
| Bo7g082150 | BGLU1-6 | EV102326 | −1.21 | 1.53 | |
| Bo7g082190 | BGLU1-6 | JCVI_25479 | −1.07 | 2.28 | |
| Bo4g098540 | BGLU7-10 | EX063486 | −2.14 | −0.70 | |
| Bo5g132800 | BGLU7-10 | JCVI_11645 | −2.51 | −0.13 | |
| Bo8g098070 | BGLU7-10 | JCVI_17890 | −1.53 | −1.80 | |
| Bo8g098080 | BGLU7-10 | JCVI_34674 | −2.29 | −0.07 | |
| Bo5g003420 | BGLU11 | CD837111 | −2.75 | −0.40 | |
| Sinapoylglucose-AT (SCPL-AT) | Bo3g042330 | SCPL8-10 | n | ||
| Bo8g100760 | SCPL8-10 | EE503308 | −1.16 | 1.14 | |
| BAHD-AT | Bo8g002690 | 3AT | n | ||
| Bo9g008920 | 5AT | n |
Enzyme steps, predicted genes and respective microarray elements with highest homology are listed. Less specific but highly homologous elements are listed repeatedly. Log2-fold changes are calculated as 100 PAR-400 PAR light intensity difference (LL-HL), or 5–15°C temperature difference (LT-HT). Significant changes are visualized, HL induced in yellow, HT induced in red, and LT induced in blue. Color intensity indicates respective expression differences. n, no microarray element with homology to the respective gene is present on the chip.