Figure 3.

PKM2 is a target of miR-133b in lung cancer cells. (A) A549 cells were transfected with 100 nM pre-miR-negative (control miR) or pre-miR-133b for 48 h (left). Cell lysates were prepared for western blotting analysis of the expression of PKM2 (right). β-actin was used as a loading control. (B) A549 cells were transfected with 100 nM anti-miR-negative (control miR) or antisense-miR-133b for 48 h (left). Cell lysates were prepared for western blotting analysis of the expression of PKM2 (right). β-actin was used as a loading control. Columns represent the mean of three independent experiments; bars represent standard error. **P<0.01 vs. control miR. miR, microRNA; PKM2, pyruvate kinase isoform M2.