Figure 5.

Overexpression of miR-133b resensitizes radioresistant lung cancer cells through the inhibition of glucose metabolism. (A) A549 radioresistant cells were transfected with 100 nM pre-miR-negative (control miR) or cotransfected with pre-miR-133b + vector control or cotransfected with pre-miR-133b + PKM2 for 48 h. Cell lysates were prepared for western blotting analysis of the expression of PKM2. Western blotting experiments demonstrated that the overexpression of PKM2 in the miR-133b-overexpressing A549 radioresistant cells restored the expression of PKM2 to a similar level to that observed in the control cells. (B) A540 radioresistant cells were transfected with control miR, miR-133b + vector and miR-133b + PKM2 for 48 h, and subsequently, cell viabilities were analyzed following radiation treatments of 0, 0.5, 1, 2, 4 and 6 Gy. Columns represent the mean of three independent experiments; bars represent the standard error. *P<0.05 and **P<0.01 vs. control miR. miR, microRNA; PKM2, pyruvate kinase isoform M2.