Figure 3.

Expression of yes-associated protein in the three orthotopic gastric cancer groups. (A) Reverse transcription-polymerase chain reaction analysis of YAP mRNA level. (B) Western blot analysis of YAP protein expression level and (C) quantification of western blot analysis. YAP expression levels were detected by immunohistochemistry in (D) CON group, (E) NC group and (F) YAP-shRNA group (all magnification, x200). Data are presented as the mean ± standard deviation. **P<0.01 vs. CON and NC groups. CON, untransfected cells; NC, negative control vector-transfected cells; YAP-shRNA, yes-associated protein small hairpin RNA vector-transfected cells; mRNA, messenger RNA; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.