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Primers used for GSTP1 gene methylation-specific polymerase chain reaction.
| Gene | M/U | Sequences of the primers (5′-3′) | Annealing temperature | Product length |
|---|---|---|---|---|
| GSTP1 | M | (F)-TTCGGGGTGTAGCGGTCGTC | 61°C | 91 bp |
| (R)-GCCCCAATACTAAATCACGACG | ||||
| U | (F)-GATGTTTGGGGTGTAGTGGTTGTT | 55°C | 91 bp | |
| (R)-CCACCCCAATACTAAATCACAACA |
M, methylated; U, unmethylated.
