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. 2016 Mar 8;11(4):2792–2800. doi: 10.3892/ol.2016.4315

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Copyright: © Yu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 1. — Identification, characterization and differentiation U87-GSCs and C6-GSCs. U87 and C6 gliospheres were cultured for 7 days, immunocytochemical staining of (A) the cancer stem cell marker, CD133, and (B) the neural progenitor marker, nestin, in U87-GSC and C6-GSC cells. (C) Following differentiation, the U87 and C6 glioma cells were immunostained with β-tubulin-III and GFAP. The fluorescent signals were detected and images were captured at x200 magnification. GSCs, glioma stem cells; GFAP, glial fibrillary acidic protein.