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Inhibition of gliosphere formation and expansion by TMZ and MET. (A) U87-GSCs and (B) C6-GSCs were cultured in the absence or presence of TMZ, MET or TMZ and MET for 7 days and the number of gliospheres counted under a microscope. The results are representative of 3 independent experiments *P<0.05, compared with control group; ∆P<0.01, compared with single agents. GSCs, glioma stem cells; TMZ, temozolomid; MET, metformin.
