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Effects of TMZ, MET or TMZ and MET on cell cycle distribution. (A) U87-GSCs and (B) C6-GSCs (2×105 cells) were cultured in the presence of 0.4 mmol TMZ, 20 mmol MET or TMZ and MET. The cells were harvested after 48 h and stained with propidium iodide (PI), and analyzed by flow cytometry. GSCs, glioma stem cells; TMZ, temozolomid; MET, metformin.
