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Purification of protease from Geobacillus toebii LBT 77.
| Purification step | Total activity (U) | Total protein (mg) | Specific activity (U/mg) | Recovery (%) | Purification fold |
|---|---|---|---|---|---|
| Crude enzyme | 11357 | 171.5 | 66.5 | 100 | 1 |
| Ammonium sulfate precipitation | 8064 | 45 | 180 | 71 | 1.5 |
| Sephadex G-75 | 2504 | 7 | 348 | 22 | 4.5 |
| DEAE-Cellulose | 2292.5 | 3 | 739.5 | 20 | 5 |
