. 2016 Mar 16;2016:8367063. doi: 10.1155/2016/8367063

Table 1.

Primer sequences for reference genes.

Gene symbol	Primer	NCBI accession numbers	PCR efficiency	Amplicon length
ACTB	F: TGACGTTGACATCCGTAAAG	NM_007393.3	90.9%	143 bp
ACTB	R: GAGGAGCAATGATCTTGATCT	NM_007393.3	90.9%	143 bp

GAPDH	F: GGTGAAGGTCGGTGTGAACG	NM_001289726.1	101.9%	233 bp
GAPDH	R: CTCGCTCCTGGAAGATGGTG	NM_001289726.1	101.9%	233 bp

18sRNA	F: GTAACCCGTTGAACCCCATT	NR_003278.3	95.1%	151 bp
18sRNA	R: CCATCCAATCGGTAGTAGCG	NR_003278.3	95.1%	151 bp

B2M	F: GCTATCCAGAAAACCCCTCAA	NM_009735.3	96.2%	300 bp
B2M	R: CATGTCTCGATCCCAGTAGACGGT	NM_009735.3	96.2%	300 bp

RPL13A	F: GAGGTCGGGTGGAAGTACCA	NM_009438.5	95.2%	71 bp
RPL13A	R: TGCATCTTGGCCTTTTCCTT	NM_009438.5	95.2%	71 bp

EF1α	F: CTGAACCATCCAGGCCAAAT	NM_010106.2	98.0%	60 bp
EF1α	R: GGCTGTGTGACAATCCAG	NM_010106.2	98.0%	60 bp

UBC	F: GCCCAGTGTTACCACCAAGA	NM_019639.4	98.6%	104 bp
UBC	R: CCCATCACACCCAAGAACA	NM_019639.4	98.6%	104 bp

HPRT1	F: CCAGCGTCGTGATTAGCG	NM_013556.2	96.3%	222 bp
HPRT1	R: CCAGCAGGTCAGCAAAGAAC	NM_013556.2	96.3%	222 bp

YWHAZ	F: CAGTAGATGGAGAAAGATTTGC	NM_001253807	97.4%	92 bp
YWHAZ	R: GGGACAATTAGGGAAGTAAGT	NM_001253807	97.4%	92 bp

TUBB	F: CGGCAACTATGTAGGGGACT	NM_178014.3	96.7%	194 bp
TUBB	R: CAGCACCACTCTGACCAAAG	NM_178014.3	96.7%	194 bp