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. 2016 Mar 30;6:23673. doi: 10.1038/srep23673

Figure 5. Response of hypothalamic astrocyte-enriched cultures to acyl-ghrelin treatment.

Figure 5

Relative levels of (A) glucose transporter (GLUT)2, (B) glucose uptake, (C) glutamate-aspartate transporter (GLAST), and (D) glutamate uptake in primary astrocyte-enriched cultures from the hypothalamus of male Wistar rats treated with acyl-ghrelin (AG) or vehicle (Ct) for 1 h or 24 h. (E) Glutamate levels in the media of primary astrocyte cultures decreased after 24 h exposure to AG. Relative levels of (F) glutamine synthetase, (G) lactate dehydrogenase, (H) monocarboxylate transporter (MCT)4, (J) glycogen phosphorylase and (K) intracellular glycogen content were measured in primary astrocyte-enriched cultures from the hypothalamus of male Wistar rats treated with acyl-ghrelin (AG) or vehicle (Ct) for 1 h or 24 h. Lactate levels (I) were measured in the media of primary astrocyte cultures after 1, 3 or 24 h exposure to AG. *p < 0.05, #p < 0.01, **p < 0.001, ***p < 0.0001, NS = not significant. Representative Western blots are from the same gel although samples were not always contiguous within the gel.