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. 2016 Mar 8;11(4):2845–2850. doi: 10.3892/ol.2016.4313

Figure 1.

Figure 1.

Expression and function of miR-187 in diffuse large B-cell lymphoma cells. (A) Relative expression level of miR-187 was detected via reverse transcription-quantitative polymerase chain reaction in B-cells from healthy donors, Raji cells, OCI-LY3 and SUDHL cells. The U6 gene was used as an endogenous control. (B) Relative expression of miR-187 in Con, SUDHL cells electroporated with 100 pM mimics or Scr negative control for 24 h. All experiments were repeated at least three times. (C) Cell viability was analyzed using CCK-8 assay in the SUDHL cells transfected with scramble or miR-187 mimics compared with the parental control. (D) Flow cytometry assay using annexin V-FITC and PI staining was utilized to detect the apoptosis of the Con cells, SUDHL cells electroporated with 100 pM mimics or Scr negative control for 48 h. (E) Western blot analysis was used to detect the cleavage activity of caspase-3 and PARP in the SUDHL2 cells with different treatment for 48 h. *P<0.05 and **P<0.01 vs. controls. miR, microRNA; Con, parental control SUDHL cells; Scr, scramble; Mimics, miR-187 mimics; FITC, fluorescein isothiocyanate; PI, propidium iodide; PARP, poly ADP ribose polymerase.