Figure 2.

miR-187 directly binds to the 3′-UTR of BCL6 mRNA. (A) miR-187 binding sites on the 3′-UTR of BCL6 mRNA had been predicted by www.microrna.org. Black font indicates the conserved binding sites. (B) Western blotting was utilized to detect the protein level of BCL6 in the B-cells from healthy donors, and the Raji, OCI-LY3 and SUDHL2 cells. GAPDH was used as an endogenous control. (C) BCL6 protein level in the SUDHL2 cells overexpressing miR-187 mimics or scramble control. GAPDH was used as a control. (D) A luciferase reporter assay was performed to detect the binding of miR-187 with the complementary fragment harbouring in the 3′-UTR of BCL6 mRNA. All experiments were repeated at least three times. *P<0.05 vs. controls. UTR, untranslated region; BCL6, B-cell lymphoma 6; miR, microRNA; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; Scr, scramble; wt, wild-type; mt, mutant-type.