Figure 1. NECA increased fluorescein extravasation and concentration in blood.

Mice received fluorescein (i.v. via tail vein) at 3 h after NECA injection (n = 6 per group), and fluorescein concentrations in forebrain (A₁), cerebellum (B₁), liver (C), and blood (D) were quantified 90 min later. BBB permeability was normalized to fluorescein concentration in blood for fluorescein concentrations in forebrain (A₂) and cerebellum (B₂). The bars represent means ± SEM. Significant differences were determined by Fisher’s LSD test. *p < 0.05, **p < 0.01, and ***p < 0.001; n.s. indicates no significant difference.