(b)  

Antigen	RMASc	Tetramer bindingd			Tolerance inductione
		3 weeks	13 weeks	Diabetic	Purified peptide	Peptide + Zymosan A	Plasmid
Control	+	−	−	0.04
Insulin	+	0.03	0.72	0.89	nt	+	+
Gad65	−	0.02	0.1	0.1	nt	nt	nt
Gad65	+	0.03	0.39	0.14	nt	ns	nt
IGRP	+	−	0.63	0.57	nt	ns	nt
ICAp69	+	−	−	−	nt	ns	nt
ZnT8158	+	−	0.20	0.26	+	ns	nt
ZnT8282	+	−	0.69	0.60	+	ns	ns
DβH	+	0.09	0.41	0.32	+	nt	ns
Ins + DβH						nt	+
Ins + ZnT8						nt	+
Ins + DβH + ZnT8						nt	+

Control peptide is influenza NP.

“−” and “+” refer to whether peptide surpasses cutoff assignment.

“nt”: not tested.

“ns”: not significant.

a: scoring cutoff is >5-fold over control.

b: scoring cutoff is >500 pg/mL. (control is <100 pg/mL).

c: scoring cutoff is >2-fold difference in MFI.

d: % of pancreatic LN CD8⁺ T cells.

e: “+” indicates scoring cutoff has statistically significant P value versus control peptide.