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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: Cell Mol Bioeng. 2015 Oct 20;9(1):38–54. doi: 10.1007/s12195-015-0423-6

Figure 1. Schematic of 3D PEG hydrogel system to support EPC microvessel formation.

Figure 1

(A) Overview of acryl-PEG-RGDS (PEG-RGDS) synthesis. (B) An MMP-sensitive peptide sequence (PQ) was incorporated into PEG diacrylate (PEGDA) through reaction with Acryl-PEG-SVA at a 1 to 2.1 molar ratio to form Acryl-PEG-PQ-PEG-Acryl (PEG-PQ). (C) Co-cultures of ECs with SMCs were mixed with PEG-PQ, PEG-RGDS, and polymerization components eosin Y, triethanolamine, and 1-vinyl-2 pyrrolidinone. Hydrogels were formed from the mixture by exposure under visible light at 120 mW/cm2 for 40s.