. Author manuscript; available in PMC: 2016 Mar 30.

Published in final edited form as: J Phys Chem B. 2016 Jan 20;120(4):633–640. doi: 10.1021/acs.jpcb.5b09040

Table 1.

Peptide Sequences of Wild Type AChR M2δ and ESEEM Experimental Constructs^a

	N-terminal (−)	C-terminal (+)
wild type	NH₂-EKMSTAISVLLAQAVFLLLTSQR-COOH
Leu10	NH₂-EKMSTAISXiLAQAVFLLLTSQR-COOH	NH₂-EKMSTAISViXAQAVFLLLTSQR-COOH
	NH₂-EKMSTAIXViLAQAVFLLLTSQR-COOH	NH₂-EKMSTAISViLXQAVFLLLTSQR-COOH
	NH₂-EKMSTAXSViLAQAVFLLLTSQR-COOH	NH₂-EKMSTAISViLAXAVFLLLTSQR-COOH
	NH₂-EKMSTXISViLAQAVFLLLTSQR-COOH	NH₂-EKMSTAISViLAQXVFLLLTSQR-COOH
Leu11	NH₂-EKMSTAISVXiAQAVFLLLTSQR-COOH	NH₂-EKMSTAISVLiXQAVFLLLTSQR-COOH
	NH₂-EKMSTAISXLiAQAVFLLLTSQR-COOH	NH₂-EKMSTAISVLiAXAVFLLLTSQR-COOH
	NH₂-EKMSTAIXVLiAQAVFLLLTSQR-COOH	NH₂-EKMSTAISVLiAQXVFLLLTSQR-COOH
	NH₂-EKMSTAXSVLiAQAVFLLLTSQR-COOH	NH₂-EKMSTAISVLiAQAXFLLLTSQR-COOH
Leu17	NH₂-EKMSTAISVLLAQAVXiLLTSQR-COOH	NH₂-EKMSTAISVLLAQAVFiXLTSQR-COOH
	NH₂-EKMSTAISVLLAQAXFiLLTSQR-COOH	NH₂-EKMSTAISVLLAQAVFiLXTSQR-COOH
	NH₂-EKMSTAISVLLAQXVFiLLTSQR-COOH	NH₂-EKMSTAISVLLAQAVFiLLXSQR-COOH
	NH₂-EKMSTAISVLLAXAVFiLLTSQR-COOH	NH₂-EKMSTAISVLLAQAVFiLLTXQR-COOH
Leu18	NH₂-EKMSTAISVLLAQAVFXiLTSQR-COOH	NH₂-EKMSTAISVLLAQAVFLiXTSQR-COOH
	NH₂-EKMSTAISVLLAQAVXLiLTSQR-COOH	NH₂-EKMSTAISVLLAQAVFLiLXSQR-COOH
	NH₂-EKMSTAISVLLAQAXFLiLTSQR-COOH	NH₂-EKMSTAISVLLAQAVFLiLTXQR-COOH
	NH₂-EKMSTAISVLLAQXVFLiLTSQR-COOH	NH₂-EKMSTAISVLLAQAVFLiLTSXR-COOH

Wild type and experimental constructs of AChR M2δ (α-helix) are listed in this table. i is the positions where ²H-labeled d₁₀-Leu was placed. X is the position for MTSL incorporation.