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. 2016 Mar 16;2016:7589813. doi: 10.1155/2016/7589813

Figure 2.

Figure 2

Resveratrol increased the expression of SIRT1 and enhanced autophagic flux in HUVECs. (a) Cell viability was determined by CCK-8 assay after treatment of HUVECs with different concentrations of resveratrol for 12 h. Western blotting analysis showed that resveratrol increased the expression of SIRT1 and LC3-II and decreased that of p62 in a dose-dependent manner (b, c). Resveratrol (50 μM) increased the expression of SIRT1 and LC3-II and decreased that of p62 in a time-dependent manner (d, e). Actin served as loading controls. Data are expressed as mean ± SEM; n = (3–7); ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001 versus controls of SIRT1; $ p < 0.05, $$ p < 0.01, and $$$ p < 0.001 versus controls of LC3II; # p < 0.05, ## p < 0.01 versus controls of p62; NS: not significant.