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. 2016 Mar 16;2016:7589813. doi: 10.1155/2016/7589813

Figure 3.

Figure 3

Resveratrol ameliorates Ox-LDL-induced impaired autophagic flux and reduces the accumulation of Ox-LDL in HUVECs. Western blotting analysis of SIRT1, LC3-II, and p62 after HUVEC pretreatment with 50 μM resveratrol for 1 h and subsequently incubation with Ox-LDL (60 μg/mL) for 12 h (a, b). Data are expressed as mean ± SEM; n = 3; ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001 versus controls; NS: not significant. (c) The accumulation of Dil-Ox-LDL in HUVECs was determined by confocal microscopy. Scale bar: 100 μm. (d) Flow cytometry detected the fluorescence density of Dil-Ox-LDL in HUVECs. (e) Western blotting analysis of LOX-1 after cells was treated with Ox-LDL or RSV; data are expressed as mean ± SEM; n = 3; ∗∗ p < 0.01 versus control; NS: not significant.