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. 2016 Mar 16;2016:7589813. doi: 10.1155/2016/7589813

Figure 5.

Figure 5

Resveratrol amelioration of autophagic flux in HUVECs in the presence of Ox-LDL is dependent on SIRT1. (a) Western blot analysis of SIRT1 in HUVECs transfected with SIRT1 or nontargeting siRNA. Pretreatment with resveratrol (50 μM) subsequently incubated with Ox-LDL (60 μg/mL) for 12 h after SIRT siRNA transfection. Western blot analysis of SIRT1, LC3-II, p62, and cathepsin D (b–f). Cathepsin D activity was detected with a cathepsin D activity assay kit (g). Data are expressed as mean ± SEM; n = 5; ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001 versus corresponding controls. Cells were transfected with SIRT1 or nontargeting siRNA for 24 h and then treated with Dil-Ox-LDL for 12 h; flow cytometry detected the fluorescence density of Dil-Ox-LDL in HUVECs (h).