Table 1.

Comparison of sensitivity of nested PCR, qPCR, nested PCR, and ddPCR in the detection of the SYT-SSX2 fusion gene in synovial sarcoma cells. D: detected, ND: not detected.

Amount of total RNA in ng (synovial sarcoma cells)	Nested qPCR	qPCR	Nested PCR	ddPCR
25,000	D	D	D	D
12,500	D	D	D	D
6,250	D	D	D	ND
3,125	D	D	D	ND
1,562	D	D	ND	ND
0,781	D	D	ND	ND
0,391	D	ND	ND	ND
0,195	D	ND	ND	ND
0,098	ND	ND	ND	ND
0,049	ND	ND	ND	ND
0,024	ND	ND	ND	ND
0,012	ND	ND	ND	ND
0,006	ND	ND	ND	ND
0,003	ND	ND	ND	ND