Figure 4.

LIF is required for norgestrel-mediated cell survival in 661W photoreceptor cells. A: Cells were maintained in complete medium without any treatment, i.e., healthy cells (cells), or were serum starved for 24 h and treated with 20 µM norgestrel (Norg) or vehicle control (ctrl). B: Before 24 h serum starvation, the cells were transfected with siRNA targeted against leukemia inhibitory factor (siLif) or a non-targeting scrambled control (scram). C: Cells were again transfected with siLif or scrambled control followed by serum starvation and treatment with 20 µM norgestrel (Norg) or vehicle control (ctrl). D: Detection of Lif knockdown with real-time (RT) PCR (left) and western blotting (right). Error bars denote $\pm$standard error of the mean (SEM) from three independent experiments. *p<0.05, **p<0.01 (ANOVA).