Figure 2.

EMT marker expression. Analysis of EMT inhibition in (A) A549 and (B) HCC4006 cells by western blotting. Cells were treated for 48 h with TGFβ (10 ng/ml) alone, or TGFβ (10 ng/ml) plus metformin or salinomycin in two different concentrations. Protein expression in the two cell lines was compared with completely untreated cells or cells treated with metformin or salinomycin only. E-cadherin expression was used as a marker of the epithelial phenotype, whilst the mesenchymal marker was vimentin. GAPDH was used as a loading control. EMT, epithelial-to-mesenchymal transition; TGFβ, transforming growth factor β.