Fig. 1.
Schematic presentation and validation of plasmid constructs. a pBI-RCP-1:GUS- the maize Root Cap-specific Protein-1 promoter (RCP1p) drives expression of the β-glucuronidase (gusA) gene. b pBI121- the cauliflower mosaic virus CaMV35S promoter (35Sp) drives expression of the gusA gene. For both constructs the nopaline synthase promoter (NOSp) was used to drive expression of the neomycin phosphotransferase II (nptII) gene and the nopaline synthase terminator (NOSt) was used as the 3΄ poly-A signal for both the selectable marker nptII and gusA genes. Left (LB) and right (RB) borders of the T-DNA and translation start sites with the direction of transcription are also indicated. c Polymerase chain reaction screen of Agrobacterium tumefaciens strain EHA105 colonies (1–9) transformed with pBI-RCP-1:GUS or pBI121. RCP1 primers amplify a 2 kbp fragment and the 35S primers amplify an 835 bp fragment. M—molecular size marker with sizes of bands indicated; P—positive control using the plasmid used for transformation