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. 2016 Mar 29;15:57. doi: 10.1186/s12934-016-0455-1

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© Ploss et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Heterogeneity of protein secretion stress in amyM-expressing cells. Time-lapse microscopy was performed of B. subtilis 168 Hm C5 (a) and 168 Hm C5 carrying plasmid pDAM coding for the secretory α-amylase AmyM (b). Strains were grown on 1.5 % agarose slides containing 25 % LB broth. Microscopy images are shown from cultures in the exponential, early stationary and stationary growth stages