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. 2016 Mar 29;15:57. doi: 10.1186/s12934-016-0455-1

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© Ploss et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Influence of degUhy32 and rok mutations on heterogeneous activity of P(amyQ)-M under different growth conditions. GFP expression levels and flow cytometry histograms are shown for B. subtilis strains 168 (black), 168degUhy32 (blue) and 168Δrok (red) carrying plasmid pDAPamy-gfp tet with the transcriptional P(amyQ)-M-gfpmut3 fusion upon growth in LB (a) or EnPressoB (b) medium. Samples were taken after 5.5, 8, 24 and 32 h of cultivation